Quant 3D Q150 User's Guide download pdf (Page 26)

Using the second cable tie, repeat steps a-d to secure the riser at the other

end of the foot.

45°

Using scissors, remove the excess material from each cable tie.

Carefully roll the ProFlex

™

System back onto its feet.

ProFlex

PCR System

Chapter 1 Getting Started

Prepare the QuantStudio

3D Digital PCR System for use

QuantStudio

3D Digital PCR System User Guide

1 2 ... 21 22 23 24 25 26 27 28 29 30 31 ... 121 122

QuantStudio 1
3D Digital PCR System 1
Contents 3
(Optional) 5
About this guide 8
Prerequisites 9
Instrument hardware 9
Instrument software 9
Instrument consumables 10
Getting Started 11
Data collection 12
Instrument filters 12
Operating the instrument 13
Prepare the QuantStudio 14
Plan the 15
Choose additional 16
Install the 17
Digital PCR 17
Instrument 17
IMPORTANT! The QuantStudio 20
The QuantStudio 21
Install the thermal 23
IMPORTANT! The ProFlex 24
PCR System 26
CAUTION HOT 27
Install the GeneAmp 29
PCR System 9700 29
IMPORTANT! The GeneAmp 30
IMPORTANT! If the GeneAmp 31
Connect the QuantStudio 34
3D Instrument to a network 34
3D AnalysisSuite 35
Software 35
Prepare Samples and Load 36
Reactions 36
Sample dilution 37
Determine the 37
Prepare the 39
X0300036 41
Load and seal the 43
IMPORTANT! Press the 45
Load chips manually 50
Digital PCR 20K 51
IMPORTANT! Allow the 53
Perform the PCR 58
08:00 AM 4/25/13 25.0°C 60
GeneAmp® PCR System 9700 60
Run UserUtilEditCreate 60
Version: 3.12 60
Materials 61
Guidelines for 62
Unload the 64
Analyze the Prepared Chips 66
Analysis workflow 67
About the 68
Analyze the chips 69
Analyzing the data 73
About the results 74
About data quality 75
About the data 75
Transfer the 76
Using the QuantStudio 77
Troubleshooting 79
(QuantStudio 82
IMPORTANT! Condensation 83
Maintenance 86
Clean the chip tray 87
Returning your QuantStudio 89
3D Instrument for service 89
Replace the instrument fuses 90
Replace the fuses 91
Update the 92
Calibrate the touchscreen 93
View the instrument log 93
Networking 95
Network setup 96
Required network 97
Connect the 98
Instrument to a 98
Create a network 103
Parts and Materials 106
Specifications and Layout 109
Instrument clearances 110
Electrical specifications 111
Environmental requirements 112
Network requirements 113
Symbols on this instrument 114
Conformity 115
Location of safety 116
Fisher Scientific 117
Instrument safety 117
3D Digital PCR Chip Loader 118
Chemical safety 119
Biological hazard safety 120
Documentation and support 121
4 February 2015 122

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Quant 3D Q150 User's Guide Page 26

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